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NET 2.5 Win CE A: I have seen this error as well. The fix was to change the clock source to network. Under Network Right Click on the interface to change clock source Scroll down to Network Select the radio button for "GPS(network)" Click OK Now the clock error should clear and you will have a synchronized time. Immunohistochemical staining for quantitative determination of the serum and follicular fluid progesterone concentration in cattle. To develop a quantitative immunohistochemical method to detect progesterone in ovarian follicles, to compare it with the progesterone RIA and to compare progesterone concentrations in serum and follicular fluid of different age groups. Double-staining immunohistochemistry was performed in cow ovaries using a monoclonal antibody against progesterone (P4), and the P4 was detected by streptavidin-biotin system using diaminobenzidine as chromogen. A comparison was made with the progesterone radioimmunoassay (RIA). The precision of the method was determined by comparing intra- and interassay variation. Histologically, the staining quality was sufficient for the comparison of the two methods. The intra- and interassay coefficients of variation of the P4 immunohistochemical method were 5.4% and 10.5%, respectively. The progesterone concentrations in serum and follicular fluid of different age groups were determined using the P4 immunohistochemical method and the progesterone RIA, and the correlation was analysed. There was a strong positive correlation between the two methods (r(s) = 0.98; P Business Directories Ashley, International Expansion and the New EBITDA